Fig. 1

Characterization of extracellular vesicles (EVs) released by HaCaT cells. a Western blotting analysis of purified vesicles (EV) for exosomal surface markers (CD9, CD63) and endoplasmic reticulum marker (GRP94) side by side with HaCaT cell lysate (CL). b Venn diagram of identified proteins and proteins in the vesiclepedia database. The pink area represents proteins from the vesiclepedia database and the blue area represents proteins we identified. The identified proteins in HaCaT cell-derived EVs were analyzed using FunRich Bioinformatic Resources and classified by c biological process, d cellular component, or e molecular function